Drivers at Avanti West Coast are to strike next month in a row over sick pay changes.
Members of the Aslef union will walkout on Sunday, July 2. The union has accused Avanti West Coast of trying to "force through without agreement".
Separately, drivers employed by London North Eastern Railway (LNER) will refuse to work overtime from July 1 until further notice.
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LNER is accused of failing to adhere to "agreed procedures".
Aslef general secretary Mick Whelan said: "It is deeply regrettable that Aslef members have been forced to take this action but our members will not stand by and allow our agreed terms and conditions to be violated by the train operating companies.
"We have been coming to the table to try to resolve these disputes for many months but unless the operators honour the agreements in place with our members, we will be forced to continue taking action.
"The companies know how to avoid this action – it’s by honouring their agreements and negotiating fairly with our members."
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Aslef announced earlier this week that its members at 15 train firms have voted overwhelmingly to continue taking industrial action for the rest of the year in the national pay dispute.
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